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Rtk Antigen Saliva Antibody Rapid Self Test Swab Kit
Product Details:
| Place of Origin: | China |
| Brand Name: | Ascentet |
| Certification: | CE FDA TGA |
| Model Number: | Covid Test Kit |
Payment & Shipping Terms:
| Minimum Order Quantity: | 10000pcs |
|---|---|
| Price: | $1.50-2.0/pcs |
| Packaging Details: | 25tests/box 1test/box |
| Delivery Time: | 5-20 days |
| Payment Terms: | T/T, Western Union, MoneyGram |
| Supply Ability: | 100000pcs/day |
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Detail Information |
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| Sample Collection:: | Nasal Swab | Package: | 25pcs/box, 1pcs/box |
|---|---|---|---|
| Use: | Fast Check Of Coronavirus | Reading Time: | In 15 Mins |
| Qualitative: | Qualitative Determination Of Covid-19 | Method: | Colloidal Gold Assay |
Product Description
15mins Reading One Step Covid POCT Covid-19 2019-NCoV Antigen Rapid Test Kit
Introduction
Covid-19 first emerged in December 2019 in Wuhan, China. The cause of the disease was confirmed as a new kind of coronavirus, this virus is transmitted through air from people to people. In two months, the infection has quickly spread in China and to a number of countries around the world.
Covid-19 virus causes over 2% mortality, there is 7 to 14 days incubation period. The current diagnostic method is majorly by PCR test, but its overall sensitivity is 50 to 60% and is limited by the user with the special trainning and special instruments. There is an urgent need to develop a simple and fast test to screen large population and unsymptomatic infected people. Infectious disease usually produces specific IgM antibody at 2 to 4 days after the infection, while specific IgG antibody appears after 5 to 7 days. Compared to other test, this test is more convenient with low cost, it can be performed in field, mobile hospital and laboratory.
Safety measure:
Covid-19 antibody test must be manipulated in a biosafety Level 3 facility, or with good personal protection, specimen deposit have to follow WHO or local health authority guidline .
Assay principle
The recombinant Covid-19 nuceloprotein antigen is used in the test, serum, plasma or whole blood from Covid-19 infected patient is loaded to the device, the specific Covid-19 antibodies in specimen will bind to gold-conjugated Covid-19 nucleoprotein antigens forming antibody – colloid gold-antigen complex. While the complex migrates along nitrocellulose membrane, the complex is captured on the test lines coated with anti-human IgM or IgG causing a pink to deep pink band at the IgG or IgM test line. The signal intensity is variable depending on the amount of specific antibody present in specimen. A red control line should always develop over control line indicating the proper performance.
Polyester sponge samples with PP (polypropylene) rods are recommended for sterile sample collection.
(1) Oropharyngeal specimen collection method: collection head slightly tilted, mouth to expose both sides of pharyngeal tonsils.Wipe the pharyngeal tonsils at least three times along the tongue root, and then wipe the posterior pharynx up and down at least three times with a cotton swab.
(2) Method of collecting nasopharyngeal specimens: The sampler gently holds the head of the recipient with one hand and holds the swab with the other hand.Plug the swab into the nostril and penetrate slowly along the bottom of the lower nasal tract.Arc, do not force too hard, to avoid trauma bleeding.When the tip of the swab reaches the posterior wall of the nasopharyngeal cavity, rotate gently once (if it is a reflex cough) and slowly remove the swab.
(3) Sample treatment: The collected samples shall be treated with the sample buffer provided by the kit as soon as possible (if not processed immediately, the samples shall be stored in dried, sterilized and sealed plastic tubes) and stored at 2℃.~ 8℃, not more than 24 hours; -70℃ is stored for a long time, but repeated freeze and thawing should be avoided.
Test Method
Prior to testing, all reagents were returned to room temperature.Testing shall be performed at room temperature.
I. Sample extraction (see Figure 1)
1. Add 400 L (about 10 drops) of sample buffer vertically to the sample extraction tube and then insert the sample into the tube and rotate against the inner wall about 10 times so that the samples in the solution dissolve as much as possible.
2. Squsample q-tip along the inner wall of the extraction tube to retain as much liquid in the tube as possible from the sample, then removed and discarded.
3. Cover the dropper.
two.Test procedure (see Figure 2)
1. Remove the test card from the sealing bag.
2. Add 2 drops (about 80 L) of the treated sample extract to the sample hole of the test card and start the timer.
3. The results were read after placing the test card for 15 minutes at room temperature.The results were invalid after 20 minutes.
Interpretation of test results
Schematic diagram of the test card result judgment:
Invalid ① result: the quality control line (C line) has no reaction line, the detection is invalid, and the test shall be repeated.
② negative result: red ribbon, quality control line (C line) is in color.
③ positive result: two red bands, detection line (T line) and quality control line (C line) are in color.